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Nanjing University, China
Title: In situ detection of cell surface glycans
Biography

Huangxian Ju, the director of State Key Laboratory of Analytical Chemistry for Life Science, received his B.S., M.S. and Ph.D degrees from Nanjing University in 1986, 1989 and 1992, and became a lecturer, associate professor and professor at Nanjing University in 1992, 1993 and 1999, respectively. He was a postdoctoral researcher in Montreal University (Canada) from 1996-1997. He won the National Funds for National Distinguished Young Scholars in 2003 and was selected as a Changjiang Professor by Education Ministry of China, a chief scientist of National Basic Research Program of China by MSTC in 2009, Fellows of the International Society of Electrochemistry and the Royal Society of Chemistry in 2015. The research interests of Professor Huangxian Ju include analytical biochemistry and nanobiosensing, focusing on signal amplification and life analytical chemistry. He has published 676 papers (621 papers in SCI journals, and 372 papers in journals with IF>5), and authored 23 patents, 4 English books, 5 Chinese books, 17 chapters for 9 Chinese and 8 English books, and 6 editorial, preface or book reviews. Up to Nov 10, 2019, his research works have 30008 citations in SCI journals (28710 by other authors) with an h-index of 90 (Google Scholar h-index 99 with 34402 citations).

Abstract

Cellular functional biomolecules have been regarded as attractive targets for biomedical research and molecular diagnostics. Our recent efforts devote to in situ analysis and highly selective detection of various cellular functional biomolecules, such as glycans, multiple glycans, protein-specific glycans and gangliosides on living cell surface, intracellular microRNA, sialyltransferase, lysosomal neuraminidase and glycosylation, telomerase and caspases. Here I will introduce our research aim, strategies and results in design of bioprobes and signal amplification strategies for in situ sensing of cell surface glycans, including one molecule-two surfaces competitive strategy, chemoselective recognition, dual-color indicator, identification of ganglioside subtypes with electrochemical, scanometric, chemiluminescent, fluorescence, and Raman spectroscopic techniques. A hierarchical coding imaging strategy, with DNA coding and decoding of protein and monosaccharides executed in fidelity to the hierarchical order of target glycoprotein, is also reported for live cell imaging of protein-specific glycoforms, which was considered as an important step in the development of a system analogous to the use of green fluorescent proteins for protein tagging.

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